INFLUENCE OF CILEMBU BULBS ON CHOLESTEROL LEVELS THE WHITE MICE (Mus musculus) IN VIVO

ABSTRACT

INFLUENCE OF CILEMBU BULBS ON CHOLESTEROL LEVELS THE WHITE MICE (Mus musculus) IN VIVO

Anis Susanti⁽¹⁾, Cikra Ikhda N.H.S ⁽²⁾, Dewy Resty B ⁽³⁾

Departement of Biology Pharmacy, IIK, Kediri, Indonesia

Email : cikranursanianto@yahoo.com

Increased levels of cholesterol in the blood is a major cause of atherosclerosis. This study aimed to examine the effect Cilembu bulbs that can lower cholesterol levels in experimental Mus musculus were induced by High Fat Diet Eat (MDLT) consisting of oil and beef fat with a ratio of  5:1 which aims to optimize cholesterol levels blood of mice. 30 research subjects mice. This experiment was divided into 6 groups: negative control (PGA 2% BB), positive control (MDLT 2% BB), comparison (2% + simvastatin MDLT 0.026 mg / 20 g BW), and 3 different doses (0.5 , 2, 5 mg / 20 g BW). Total blood cholesterol levels of tail measured by Glico test. Data were analyzed by repeated ANOVA, followed by Duncan test. The tubers Cilembu significant effect on lowering blood cholesterol levels (p <0,05).

Results of this study showed that infusion at a dose of 0.5 Cilembu bulbs, 2, 5 mg/20 g BW mice can lower total cholesterol. Decrease in total cholesterol levels by 21,27% concentration can lower total cholesterol levels were the highest obtained in mice given Cilembu tuber infusion at a dose of 5 mg/20 g BW.

Keywords: bulbs Cilembu, total cholesterol, Mus musculus

Blood glucose and total cholesterol content of hyperglicemic white mice (Mus musculus) after orally intakes Juice of (Citrus aurantifolia Swingle)

ABSTRACT

Blood glucose and total cholesterol content of hyperglicemic white mice (Mus musculus) after orally intakes Juice of (Citrus aurantifolia Swingle)

Fridyani Mita R⁽¹⁾, Cikra Ikhda N.H.S.,S.Farm, M.Si, Apt⁽²⁾, Dewy Resty B.,S.Farm, Apt⁽³⁾

Departement of Pharmaceutical Biology-IIK-Kediri-Indonesia

Email : Cikranursanianto.yahoo.com

The main symtomp of diabetes mellitus is an increase of blood glucose concentration, therefore it can be decreased using natural subtances. The research aimed to the juice of Citrus aurantifolia Swingle can decrease the concentration of blood glucose and total cholesterol content in Mus musculus. The subjects were 25 Mus musculus of BalB/cAnN starin. The chemical subtance used to increase the concentration of blood glucose  was 1 cc/kb BB of dextrose monohidrat 10%. The animals were devided into 6 groups that are threaded by giving : negative control (CMC 1%, 1 ml/20 g BB), positiv control (glibenclamid 0,013 mg/20 g BB), juice of Citrus auratifolia Swingle in various concentration (0,5, 2, 5 mg/20 g BB). The concentration of blood  glucose and total cholesterol content was measured using glico test. The data gained were analyzed using repetead ANOVA followed by Duncan at 5% confidence level. The results of this research shows that the of Citrus aurantifolia Swingle giving significant effect on the reduction of blood glucose (p<0,05), however it does not appear to have the same result to total cholesterol content. At various concentration of Citrus aurantifolia Swingle, the total cholesterol of rat remain stable. The optimum concentration to provide hypoglicemic activity raised at 2 mg/ 20 g BB dose.

Keywords : Citrus aurantifolia Swingle, blood glucose, total cholesterol content.

QUALLITY OF LIFE MEASUREMENT IN HEART FAILURE PATIENTS THERAPY AND TOTAL COST MEDICATION

QUALLITY OF LIFE MEASUREMENT IN HEART FAILURE PATIENTS THERAPY AND TOTAL COST MEDICATION

Fita ^1)*, Cikra ^2)*

InstitutIlmuKesehatan, Kediri, Indonesia

email : cikranursanianto@yahoo.com

ABSTRACT

Heart failureis a complex syndrome associated with increased mortality, hospitalization, and poor quality of life. This research was conducted to assessthe quality of life heartfailure patients with patients conditions.

In this study was involved 28 people with heart failure are treated in hospitals. Evaluation quality of life heart failure patients using a method  Heart Relation Quality of Life (HRQoL), the anallyzed among Heart Relation Quality of Life (HRQoL) with socio-demographic, degree of severity byNew York Heart Association (NYHA), the characteristics of therapy, and total cost of treatment was anallyzed using spearmank corellation that was continued bivariateand multivariate anallyzed between the independent variables.

The results of demographic characteristics the study showed that most patients with heart failure  the research subjectsbe male(67,9%), agerange45-59years(9%), education level of elementary school(60,7%), work that dominates the study subjects were farmers(39.3%). The degree severity ofNYHA most  the subjects included in the NYHA class III(60.7%). The therapy that gotten show suitable (64,3%) with the terapy characteristics of the most commonly used diuretic-furosemide(25.8%). The combination therapyof heart failure is the most widely useddrug in combination with 3 heart failure are Diuretics-Nitrates-Digoxinas many(13.3%), and a combination of 4 drugs, namely heart  failure Diuretics-Aldosteroneantagonists-ARB-Inotropic as many (13,3%). The total cost of treatment heart failure patients with comorbid greater costs(13,3%) with total costis Rp 2.151.194±30.361. The corellation anallyze show that education, gender, and severity by NYHAis an independent variable affects the quality of life heart failure patients.

Key word : HRQoL, heart failure, therapy, total cost

Panduan Risbin Iptekdok 2014

Panduan lengkap dapat di download di sini

Abstract : COMPARISON OF REDUCTION BLOOD GLUCOSE LEVELS OF LEAVES AND PULP INDONESIAN Manilkara zapota (L.)

COMPARISON OF REDUCTION BLOOD GLUCOSE LEVELS OF LEAVES AND PULP INDONESIAN Manilkara zapota (L.)

Maria Platonia*, Nuril Anwari**, Cikra INHS ***

* Faculty of Pharmacy, Institut Ilmu Kesehatan Bhakti Wiyata Kediri, Indonesia ,

** Faculty of Pharmacy, Institut Ilmu Kesehatan Bhakti Wiyata Kediri, Indonesia ,

*** *Departement of Biology Pharmacy, Faculty of Pharmacy, Institut Ilmu Kesehatan Bhakti Wiyata Kediri, Indonesia ,

E-mail : Cikranursanianto@yahoo.com

Abstract

            Indonesian Manilkara zapota (sawo) is a tropical plant that generally grows various regions in Indonesia. Based on the last research, Manilkara zapota leaves potential as an antioxidant and antihyperglycemic. Indonesian Manilkara zapota (sawo) fruit is often consumed in everyday and have glucose content. The objective of this research is to determine the potential hypoglycemic effect of  both leaves or pulp Indonesian Manilkara zapota and then compare blood glucose level after induced with dextrose 10%.

The method research was real experiment. Extracted leaves using Soxhletasi  with ethanol and the pulp using juice made ​​with each concentration of and 0.5g/kg b.w., 1.3 g/kg b.w., 10 g/kg b.w., 20 g/kg b.w., 30 g/kg b.w. Experimental animals were divided into five groups (n = 5). Group I (negative control), group II (fruit juice 1.3 g/kg b.w), III (fruit juice 0.5 g/kg b.w), IV (leaf extract 0.5 g/kg b.w) and V  (leaf extract 1.3 g / kg b.w). The data is taken two hours after giving of the extract and then every 60 minutes for 4 hours. Analized this comparison using T-Test (SPSS Vers 17,0).  

Based on study showing different content of total protein, fenolik and flavonoid of the leaves and pulp. From the calculation of blood glucose level percentage administration significant difference between group. Dose 0,5 g/kg b.w of The Leaves and pupl not showing significant difference with blood glucose lowering effect. The result this research that antihyperglycemic activity of  both leaves or pulp start from dose 1,3 g / kg b.w have effective effect to decreased blood glucose. From this research knowing that dose of leaves  1,3 g/kg b.w have blood glucose lowering effect than pulp (p<0.05). At dose 30 g/kg b.w the pulp decreased blood glucose 83% while the leaves only give 22%. So at dose 30 g/kg b.w of pulp have blood glucose higher effect than leaves at the same dose.

Keyword :  Indonesian  Manilkara zapota, Hyperglycemic, leaves, pulp